4LGT
Crystal structure of the catalytic domain of RluB in complex with a 21-nucleotide RNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115869 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.940, 42.200, 169.430 |
| Unit cell angles | 90.00, 102.61, 90.00 |
Refinement procedure
| Resolution | 40.461 - 1.300 |
| R-factor | 0.1735 |
| Rwork | 0.173 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lab |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.044 | 0.854 |
| Number of reflections | 126732 | |
| <I/σ(I)> | 13.5 | 1.14 |
| Completeness [%] | 92.0 | 85.1 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.3 | 16% (v/v) polypropylene glycol 400, 12% (v/v) 1-propanol, VAPOR DIFFUSION, HANGING DROP, temperature 293.3K |
