4LGE

Crystal structure of clAP1 BIR3 bound to T3261256

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Detector technology	CCD
Collection date	2010-06-27
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	30.520, 72.805, 116.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.147 - 1.550
R-factor	0.16413
Rwork	0.163
R-free	0.18199
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.321
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0025)

Data quality characteristics

	Overall
Low resolution limit [Å]	28.147
High resolution limit [Å]	1.550
Number of reflections	45061

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	2.5M NaCl,1OOmM Tris,0.2M MgCl2, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K