4LFU
Crystal structure of Escherichia coli SdiA in the space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Detector technology | CCD |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.284, 68.691, 69.278 |
| Unit cell angles | 90.00, 126.47, 90.00 |
Refinement procedure
| Resolution | 29.240 - 2.260 |
| R-factor | 0.21573 |
| Rwork | 0.213 |
| R-free | 0.27564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.411 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 15672 | |
| <I/σ(I)> | 14.7 | 2 |
| Completeness [%] | 95.9 | 78.1 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 287 | 0.1M 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, 0.2M Lithium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
