4LFK

Crystal Structure of D-galactose-6-phosphate isomerase in a substrate-free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97932
Spacegroup name	P 21 21 2
Unit cell lengths	108.174, 115.926, 54.858
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.960
Rwork	0.205
R-free	0.21160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nn4
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.990
High resolution limit [Å]	1.960	5.320	1.960
Rmerge	0.073	0.045	0.319
Number of reflections	50182
<I/σ(I)>	11.2
Completeness [%]	99.7	99.7	94.1
Redundancy	14.5	13.6	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.2M potassium formate, VAPOR DIFFUSION, HANGING DROP, temperature 295K