4LDV
Crystal structure of the DNA binding domain of A. thailana auxin response factor 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.362, 126.256, 83.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.080 - 1.450 |
| R-factor | 0.21101 |
| Rwork | 0.209 |
| R-free | 0.24344 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SeMet derivative |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.080 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.093 | 0.580 |
| Number of reflections | 81768 | |
| <I/σ(I)> | 7.3 | 1.4 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.75 l of 4mg/mL ARF1DBD + 0.75 l crystallization buffer (2.9 M NaFormate, 0.1 M MES 6.0, 50 mM KI), pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
