4LDN
Crystal structure of a putative purine nucleoside phosphorylase from Vibrio fischeri ES114 (Target NYSGRC-029521)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-18 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.979310 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 163.115, 163.115, 45.339 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.840 - 1.480 |
| R-factor | 0.1636 |
| Rwork | 0.162 |
| R-free | 0.18700 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.636 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX (C) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.112 | 0.801 |
| Number of reflections | 37977 | |
| <I/σ(I)> | 23.7 | 3.7 |
| Completeness [%] | 99.7 | 96.8 |
| Redundancy | 14.8 | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 298 | Protein (20mM HEPES pH7.5, 150mM NaCl, 5% glycerol, and 5mM DTT), Reservoir (MCSG2 #05 - 0.2 M Lithium Sulfate, 0.1 M CAPS:NaOH pH 10.5, 1.2 M NaH2PO4/0.8 M K2HPO4), Cryoprotection (33% Ethylene glycol), VAPOR DIFFUSION, SITTING DROP, temperature 298K |
