4LDM

Crystal Structure of an RNA-free VP40 Octameric Ring

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	85
Detector technology	CCD
Collection date	2012-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 4 2 2
Unit cell lengths	80.457, 80.457, 47.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.543 - 1.850
R-factor	0.1873
Rwork	0.186
R-free	0.20210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.360
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1150))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.457
High resolution limit [Å]	1.850	1.850
Number of reflections	13881
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M bicine, 15% PEG20000, 3% dextran sulfate sodium salt (Mr = 5 kDa), pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K