4LDI

Low resolution structure of Ebola virus M241R mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	85
Detector technology	PIXEL
Collection date	2012-01-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97945
Spacegroup name	P 41 21 2
Unit cell lengths	143.895, 143.895, 82.812
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.791 - 4.150
R-factor	0.3144
Rwork	0.312
R-free	0.37480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.403
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1150))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.749
High resolution limit [Å]	4.150	4.150
Number of reflections	6895
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris, 20% PEG3350, 5% dextran sulfate sodium salt (Mr = 5 kDa), pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K