4LDC
Crystal Structure of DOC2B C2B domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.802, 40.091, 36.922 |
| Unit cell angles | 90.00, 105.47, 90.00 |
Refinement procedure
| Resolution | 36.448 - 1.264 |
| R-factor | 0.1278 |
| Rwork | 0.127 |
| R-free | 0.14420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.106 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8_1069) |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.280 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Number of reflections | 33561 | |
| Completeness [%] | 92.2 | 29.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 292 | 22.5% isopropanol, 20% PEG 4000, 0.1 M Na-citrate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
