4LD9

Crystal structure of the N-terminally acetylated BAH domain of Sir3 bound to the nucleosome core particle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	77
Detector technology	PIXEL
Collection date	2013-05-25
Detector	PSI PILATUS 6M
Wavelength(s)	0.96863
Spacegroup name	P 61
Unit cell lengths	105.513, 105.513, 488.225
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.997 - 3.306
R-factor	0.2405
Rwork	0.238
R-free	0.29460
RMSD bond length	0.011
RMSD bond angle	1.597
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.200
Number of reflections	44335
Completeness [%]	90.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293.15	50 mM MES pH 6.5, 12% PEG 400, 12 mM MnCl2, 100 mM NaCl, 10 mM EDTA, VAPOR DIFFUSION, temperature 293.15K