4LD8

Crystal Structure of Dimeric Sudan Virus VP40

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	85
Detector technology	CCD
Collection date	2010-04-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97929
Spacegroup name	C 1 2 1
Unit cell lengths	63.364, 91.494, 48.021
Unit cell angles	90.00, 96.08, 90.00

Refinement procedure

Resolution	33.034 - 1.830
R-factor	0.1841
Rwork	0.183
R-free	0.21140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.184
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1389))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.034
High resolution limit [Å]	1.830	1.830
Number of reflections	23880
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	25% PEG400, 0.2 M magnesium chloride, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K