4LD7
Crystal structure of AnaPT from Neosartorya fischeri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99996 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.550, 242.590, 145.320 |
| Unit cell angles | 90.00, 89.99, 90.00 |
Refinement procedure
| Resolution | 39.950 - 2.830 |
| R-factor | 0.20251 |
| Rwork | 0.203 |
| R-free | 0.20211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.849 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Number of reflections | 160059 | |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES/imidazole, pH 6.5, 18-22% v/v ethylene glycol, 9-11% w/v PEG8000, 80-160 mM alcohol mix, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
