4LD6

PWWP domain of human PWWP Domain-Containing Protein 2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-06-07
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	32.148, 41.055, 77.972
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.290 - 1.700
R-factor	0.1694
Rwork	0.168
R-free	0.19470
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.384
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.049	0.291
Number of reflections	11827
<I/σ(I)>	56.4	3.7
Completeness [%]	99.0	87.3
Redundancy	11.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	25% PEG3350, 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	25% PEG3350, 0.2 M ammonium acetate, 0.1 M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K