4LC3
X-ray crystal structure of a putative UDP-4-amino-4-deoxy-l-arabinose--oxoglutarate aminotransferase from Burkholderia cenocepacia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.930, 58.910, 83.080 |
| Unit cell angles | 90.00, 97.39, 90.00 |
Refinement procedure
| Resolution | 47.380 - 1.600 |
| R-factor | 0.113 |
| Rwork | 0.111 |
| R-free | 0.14790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mdo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.407 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 | |
| High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
| Rmerge | 0.063 | 0.023 | 0.371 |
| Number of reflections | 117470 | 1395 | 8522 |
| <I/σ(I)> | 15.62 | 39.16 | 3.58 |
| Completeness [%] | 97.2 | 96.3 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 20% PEG6000, 100 mM sodium citrate tribasic, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
