4LC1
MeaB, A Bacterial Homolog of MMAA, Bound to GDP and crystallized in the presence of GDP and [AlF4]-
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03262 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.667, 78.623, 69.622 |
| Unit cell angles | 90.00, 108.61, 90.00 |
Refinement procedure
| Resolution | 47.890 - 1.800 |
| R-factor | 0.18127 |
| Rwork | 0.179 |
| R-free | 0.21652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qm7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.483 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.393 | |
| Number of reflections | 60064 | |
| <I/σ(I)> | 4.43 | |
| Completeness [%] | 99.5 | 97.7 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % (w/v) PEG3350, and 0.2 M sodium sulfate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
