4LB5

Crystal structure of PKZ Zalpha in complex with ds(CG)6 (hexagonal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	70
Detector technology	PIXEL
Collection date	2012-09-29
Detector	PILATUS 6M-F
Wavelength(s)	0.97
Spacegroup name	P 61 2 2
Unit cell lengths	56.860, 56.860, 218.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.300 - 2.000
R-factor	0.1915
Rwork	0.188
R-free	0.22430
Structure solution method	PDB ENTRY 1QBJ
RMSD bond length	0.006
RMSD bond angle	1.074
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.530
Number of reflections	15007
<I/σ(I)>	13.3	3.7
Completeness [%]		100
Redundancy	7.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	1.7 M ammonium sulfate, 0.1 M sodium acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K