4LAQ
Crystal structure of a therapeutic single chain antibody in the free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 88.340, 88.340, 88.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.090 - 2.800 |
| R-factor | 0.21021 |
| Rwork | 0.208 |
| R-free | 0.26988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.573 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.030 |
| High resolution limit [Å] | 2.380 |
| Rmerge | 0.820 |
| Number of reflections | 7243 |
| <I/σ(I)> | 42.5 |
| Completeness [%] | 99.6 |
| Redundancy | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 287.15 | 1.6M ammonium sulfate, 240 mM imidazole malate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 287.15K |
