4LAN
Crystal structure of Cordyceps militaris IDCase H195A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-26 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.9789 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.920, 56.440, 105.704 |
| Unit cell angles | 90.00, 105.41, 90.00 |
Refinement procedure
| Resolution | 36.320 - 1.750 |
| R-factor | 0.1568 |
| Rwork | 0.156 |
| R-free | 0.17940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hk5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.987 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.057 | 0.038 | 0.297 |
| Number of reflections | 82427 | ||
| <I/σ(I)> | 20.5 | ||
| Completeness [%] | 98.8 | 93.8 | 98.1 |
| Redundancy | 3.8 | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 5.6 | 289 | 30% polyethylene glycol 4000, 0.2M NH4Ac, 0.1M sodium citrate, pH 5.6, sitting drop, temperature 289K |
