4LA9
Crystal structure of an empty substrate binding domain 1 (SBD1) OF ABC TRANSPORTER GLNPQ from lactococcus lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.090, 55.500, 56.010 |
| Unit cell angles | 93.35, 92.92, 97.50 |
Refinement procedure
| Resolution | 34.729 - 1.300 |
| R-factor | 0.1579 |
| Rwork | 0.156 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.315 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 91547 | |
| Completeness [%] | 89.1 | 87.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 281 | 40% PEG600, 100 mM Sodium phosphate dibasic/Citric acid, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
