4L9O
Crystal Structure of the Sec13-Sec16 blade-inserted complex from Pichia pastoris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.10 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.509, 49.320, 90.904 |
Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
Resolution | 44.419 - 1.600 |
R-factor | 0.1744 |
Rwork | 0.173 |
R-free | 0.20460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jrp |
RMSD bond length | 0.011 |
RMSD bond angle | 1.318 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.610 |
High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
Rmerge | 0.084 | 0.051 | 0.680 |
Number of reflections | 82989 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.3 | 99.9 | 93.2 |
Redundancy | 6.9 | 7.3 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 23% (w/v) PEG 3350, 200 mM calcium chloride, 200 mM sodium thiocyanate, 100 mM Tris buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |