4L9O
Crystal Structure of the Sec13-Sec16 blade-inserted complex from Pichia pastoris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.509, 49.320, 90.904 |
| Unit cell angles | 90.00, 111.70, 90.00 |
Refinement procedure
| Resolution | 44.419 - 1.600 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.20460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jrp |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.318 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 3.340 | 1.550 |
| Rmerge | 0.084 | 0.051 | 0.680 |
| Number of reflections | 82989 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.3 | 99.9 | 93.2 |
| Redundancy | 6.9 | 7.3 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 23% (w/v) PEG 3350, 200 mM calcium chloride, 200 mM sodium thiocyanate, 100 mM Tris buffer, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
