4L95
Crystal structure of gamma glutamyl hydrolase (H218N) from zebrafish
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-07-25 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 226.505, 61.341, 156.956 |
| Unit cell angles | 90.00, 101.37, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.340 |
| R-factor | 0.24273 |
| Rwork | 0.239 |
| R-free | 0.30407 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9x |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.671 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Number of reflections | 88182 | |
| Completeness [%] | 98.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 291 | 10%(w/v) PEG 8000, 0.2M NaCl, 0.1M Na/K buffer, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
