4L8Y
Crystal structure of gamma-glutamyl hydrolase (C108A) from zebrafish
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.586, 206.505, 65.198 |
| Unit cell angles | 90.00, 113.42, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.970 |
| R-factor | 0.20177 |
| Rwork | 0.199 |
| R-free | 0.25779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9x |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.081 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.000 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Number of reflections | 87110 | |
| Completeness [%] | 99.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 20%(w/v) PEG 8000, 0.2M magnesium chloride, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
