4L8T

Structure of the Cargo Binding Domain from Human Myosin Vc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-01
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.459000
Spacegroup name	P 41 2 2
Unit cell lengths	116.399, 116.399, 114.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.068 - 2.950
R-factor	0.2247
Rwork	0.222
R-free	0.27420
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.754
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	90.000
High resolution limit [Å]	2.950
Rmerge	0.130
Number of reflections	17097
<I/σ(I)>	12.49
Completeness [%]	99.4
Redundancy	9.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	291.15	0.1M CAPS, 2M NH4SO4, 0.2M Li2SO4, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 291.15K