4L8S
Crystal structure of a human Valpha7.2/Vbeta13.3 MAIT TCR in complex with bovine MR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03316 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.068, 87.285, 155.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.039 - 2.900 |
| R-factor | 0.2305 |
| Rwork | 0.228 |
| R-free | 0.27760 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.750 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 27400 | |
| <I/σ(I)> | 20.9 | 2.9 |
| Completeness [%] | 95.8 | 97.2 |
| Redundancy | 6.3 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M Hepes, 1.7 M Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
