4L7Q

Crystal structure of gamma glutamyl hydrolase (wild-type) from zebrafish

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	110
Detector technology	CCD
Collection date	2011-10-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	271.610, 62.050, 123.940
Unit cell angles	90.00, 95.41, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.23
Rwork	0.198
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l9x
RMSD bond length	0.023
RMSD bond angle	1.910
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	X-PLOR
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	117219
Completeness [%]	97.6	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	30% PEG 4000, 0.1M Sodium citarte tribasic, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	30% PEG 4000, 0.1M Sodium citarte tribasic, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K