4L7M
Crystal structure of the archaeal HEAT-like repeats protein TON_1937 from Thermococcus onnurineus NA1
Replaces: 3B2AExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.911, 42.445, 77.215 |
| Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
| Resolution | 28.500 - 2.002 |
| R-factor | 0.2036 |
| Rwork | 0.202 |
| R-free | 0.23580 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.075 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 38857 | |
| <I/σ(I)> | 26.5 | 2.7 |
| Completeness [%] | 94.0 | 85.1 |
| Redundancy | 4.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 100mM Tris-HCl, pH 8.0, 0.8M ammonium acetate, 8% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
