4L76
Ca2+-bound E212Q mutant MthK RCK domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 118.884, 118.884, 356.052 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.563 - 2.992 |
| R-factor | 0.2198 |
| Rwork | 0.217 |
| R-free | 0.26260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aef |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.979 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 45.000 | 3.110 |
| High resolution limit [Å] | 2.992 | 6.460 | 2.992 |
| Rmerge | 0.167 | 0.079 | |
| Number of reflections | 30884 | ||
| <I/σ(I)> | 4.2 | ||
| Completeness [%] | 99.4 | 99.4 | 100 |
| Redundancy | 16.7 | 16.7 | 17.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 8% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
