4L75
Ca2+-bound D184N mutant MthK RCK domain at 2.4 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 119.228, 119.228, 353.958 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.593 - 2.393 |
R-factor | 0.1975 |
Rwork | 0.196 |
R-free | 0.24510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aef |
RMSD bond length | 0.007 |
RMSD bond angle | 1.016 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.000 | 45.000 | 2.490 |
High resolution limit [Å] | 2.393 | 5.170 | 2.393 |
Rmerge | 0.084 | 0.044 | |
Number of reflections | 59525 | ||
<I/σ(I)> | 6.5 | ||
Completeness [%] | 99.8 | 99 | 100 |
Redundancy | 16.6 | 17.6 | 10.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 7% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.3 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |