4L74
Ca2+-bound MthK RCK domain at 1.9 Angstrom with single ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.233, 38.424, 96.821 |
| Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
| Resolution | 14.842 - 1.841 |
| R-factor | 0.2128 |
| Rwork | 0.211 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aef |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.000 | 15.000 | 1.920 |
| High resolution limit [Å] | 1.841 | 3.960 | 1.841 |
| Rmerge | 0.068 | 0.036 | 0.336 |
| Number of reflections | 35686 | ||
| <I/σ(I)> | 12.3 | ||
| Completeness [%] | 96.7 | 98.9 | 96.1 |
| Redundancy | 4.4 | 4.4 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 6% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 0.2 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
