4L73
Ca2+-bound MthK RCK domain at 2.5 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 67.960, 67.960, 159.891 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.506 - 2.500 |
R-factor | 0.1808 |
Rwork | 0.178 |
R-free | 0.22550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aef |
RMSD bond length | 0.008 |
RMSD bond angle | 1.084 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.506 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.403 | |
Number of reflections | 14924 | |
<I/σ(I)> | 2.6 | |
Completeness [%] | 97.4 | 89.3 |
Redundancy | 5 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 12% PEG4000, 1 M ammonium formate, 0.1 M sodium acetate, pH 4.8, 1 M calcium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |