4L6Y

Structure of the microtubule associated protein PRC1 (Protein Regulator of Cytokinesis 1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	60.660, 209.800, 113.410
Unit cell angles	90.00, 105.64, 90.00

Refinement procedure

Resolution	29.906 - 3.301
R-factor	0.2762
Rwork	0.275
R-free	0.30640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l3i
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER ((phenix))
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	39522
Completeness [%]	90.5	82.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	294	Concentration: 5-10 mg/ml. Protein buffer: 80 mM PIPES (pH 6.8), 1 mM MgCl2, 1 mM EGTA, 150 mM KCl, VAPOR DIFFUSION, HANGING DROP, temperature 294K