4L65

Crystal structure of the Candida albicans Methionine Synthase in complex with 5-Methyl-Tetrahydrofolate and Methionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97648
Spacegroup name	P 21 21 21
Unit cell lengths	76.926, 98.940, 100.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.520 - 2.310
R-factor	0.20892
Rwork	0.205
R-free	0.27515
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.572
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.520	2.370
High resolution limit [Å]	2.300	2.330
Rmerge	0.066	0.549
Number of reflections	34134
<I/σ(I)>	11
Completeness [%]	100.0	99.8
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	50 mM NaI, 27% (w/v) PEG 3350, 0.25 mM DTT, 0.15 mM ZnSO4, 5 mM 5-Methyl-Tetrahydrofolate-Glu3, 10 mM Methionine, 20 mM Tris-Cl pH 7.4 , VAPOR DIFFUSION, HANGING DROP, temperature 293K