4L5G

Crystal structure of Thermus thermophilus CarD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97920
Spacegroup name	C 2 2 21
Unit cell lengths	75.128, 136.085, 104.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.862 - 2.390
R-factor	0.2018
Rwork	0.200
R-free	0.22970
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.862	2.490
High resolution limit [Å]	2.390	2.390
Number of reflections	40936
Completeness [%]	99.9	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	100 mM Bis-Tris, pH 6.5, 6% ethylene glycol, 30% (v/v) 1,3-butanediol, VAPOR DIFFUSION, HANGING DROP, temperature 298K