4L5F
Crystal Structure of DENV1-E106 Fab bound to DENV-1 Envelope protein DIII
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-08-01 |
Detector | NOIR-1 |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.661, 91.823, 92.599 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.998 - 2.450 |
R-factor | 0.1921 |
Rwork | 0.190 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.822 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.540 |
High resolution limit [Å] | 2.450 | 5.250 | 2.450 |
Rmerge | 0.083 | 0.051 | 0.546 |
Number of reflections | 26014 | ||
<I/σ(I)> | 13.8 | ||
Completeness [%] | 99.5 | 100 | 95.9 |
Redundancy | 6.4 | 6.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 293 | 14 mg/mL in a well solution of 22% PEG 6000, and 0.1 M MES pH 5.0 (final pH 5.7), VAPOR DIFFUSION, HANGING DROP, temperature 293K |