4L54

Structure of cytochrome P450 OleT, ligand-free

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 43 21 2
Unit cell lengths	59.710, 59.710, 245.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.000 - 2.300
R-factor	0.20034
Rwork	0.197
R-free	0.25596
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.018
RMSD bond angle	1.561
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	41.000
High resolution limit [Å]	2.300
Number of reflections	19580
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2 M magnesium chloride 0.1 M Tris, 25% w/v PEG 2K MME, VAPOR DIFFUSION, SITTING DROP, temperature 277K, pH 8.5