4L3F
Crystal structure of Internalin K (InlK) from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.461 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.100, 89.500, 186.300 |
| Unit cell angles | 90.00, 103.50, 90.00 |
Refinement procedure
| Resolution | 47.900 - 2.390 |
| R-factor | 0.1958 |
| Rwork | 0.192 |
| R-free | 0.23239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.800 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 92217 | |
| Completeness [%] | 81.4 | 36.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | 18% PEG3350, 0.2 M magnesium nitrate, 20 mM cesium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
