4L3A
Crystal structure of Internalin K (InlK) from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.8443 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 115.340, 115.340, 190.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.347 - 2.591 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.24310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.602 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.347 | 2.740 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 38753 | |
| Completeness [%] | 85.4 | 50 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 18% PEG3350, 0.3 M magnesium chloride, 0.1 mM samarium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
