4L2D

X-ray structure of the Fe(II) form of the iron superoxide dismutase from Pseudoalteromonas haloplanktis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.303, 103.625, 89.339
Unit cell angles	90.00, 103.68, 90.00

Refinement procedure

Resolution	29.200 - 2.070
R-factor	0.2
Rwork	0.200
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.200	2.140
High resolution limit [Å]	2.070	2.070
Number of reflections	53308
Completeness [%]	98.2	85.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ammonium sulphate 2.0 M, NaCl 0.1 M, Hepes 0.1 M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K