4L22

Crystal structure of putative glycogen phosphorylase from Streptococcus mutans

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-01-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979142
Spacegroup name	C 1 2 1
Unit cell lengths	119.720, 85.020, 84.460
Unit cell angles	90.00, 93.30, 90.00

Refinement procedure

Resolution	84.320 - 2.450
R-factor	0.22385
Rwork	0.222
R-free	0.26597
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c4m
RMSD bond length	0.011
RMSD bond angle	1.119
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall
Low resolution limit [Å]	84.320
High resolution limit [Å]	2.450
Number of reflections	38983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Solid-liquid interface method	8.5	289	0.2M (NH4)2SO4, 0.1M Tris, 25% PEG3350, pH 8.5, Solid-liquid interface method, temperature 289K