4L1R
Glycoprotein B from Herpes Simplex Virus type 1, A549T Rate-of-Entry mutant, low-pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 3 |
| Unit cell lengths | 117.432, 117.432, 160.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.473 - 3.026 |
| R-factor | 0.2028 |
| Rwork | 0.201 |
| R-free | 0.24330 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.877 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1391) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.130 |
| High resolution limit [Å] | 3.020 | 6.500 | 3.020 |
| Rmerge | 0.118 | 0.048 | |
| Number of reflections | 48077 | ||
| <I/σ(I)> | 6 | ||
| Completeness [%] | 99.6 | 100 | 96 |
| Redundancy | 7.9 | 8.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 15% PEG 4000, 0.3 M NaCl, 0.1 M Sodium Citrate, pH 5.5, vapor diffusion, hanging drop, temperature 296K |
