4L1P
Crystal Structure of Human Rtf1 Plus3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-30 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.636, 68.025, 116.972 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.158 - 2.120 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u1u |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.905 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.160 |
| High resolution limit [Å] | 2.120 | 5.750 | 2.120 |
| Rmerge | 0.085 | 0.036 | 0.624 |
| Number of reflections | 14681 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 91.9 | 85.7 | 94.1 |
| Redundancy | 4.6 | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Trisodium citrate, PEG 3000, pH 5.5, vapor diffusion, sitting drop, temperature 293K |
