4L12
Crystal structure of EGFP-based Calcium Sensor CatchER complexed with Gd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.062, 88.334, 118.167 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.130 - 1.780 |
| R-factor | 0.1972 |
| Rwork | 0.196 |
| R-free | 0.21965 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2okw |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.594 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.085 | 0.185 |
| Number of reflections | 30260 | |
| <I/σ(I)> | 14.2 | 8.7 |
| Completeness [%] | 98.3 | 93.8 |
| Redundancy | 7.2 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 51 mM HEPES, pH 7.0, 1 mM beta-mercaptoethanol, 50 mM sodium acetate, 17% PEG4000, 2 mM gadolinium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
