4L07
Crystal structure of the maleamate amidase Ami from Pseudomonas putida S16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-21 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 133.489, 133.489, 88.844 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.750 |
| R-factor | 0.1991 |
| Rwork | 0.198 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.138 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 3.770 | 1.750 |
| Rmerge | 0.079 | 0.040 | 0.492 |
| Number of reflections | 178457 | ||
| <I/σ(I)> | 11.7 | ||
| Completeness [%] | 99.9 | 99.5 | 100 |
| Redundancy | 11.4 | 11.5 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 1.0M (NH4)2SO4, 0.1M HEPES, pH 7.0, 0.5% PEG8000, vapor diffusion, hanging drop, temperature 287K |
