4KZS
Crystal structure of the secreted protein HP1454 from the human pathogen Helicobacter pylori
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97887, 0.97826 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.640, 101.350, 121.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.440 - 2.700 |
| R-factor | 0.25043 |
| Rwork | 0.248 |
| R-free | 0.30433 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.982 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.540 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.092 | 0.609 |
| Number of reflections | 12784 | |
| <I/σ(I)> | 14.3 | 3.3 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 9.1 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 298 | 0.1 M Sodium Acetate, and 2.0 M Ammonium Sulfate, pH 4.6, VAPOR DIFFUSION, temperature 298K |
