4KZJ
Crystal Structure of TR3 LBD L449W Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.402, 76.600, 127.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.160 - 2.120 |
| R-factor | 0.2142 |
| Rwork | 0.212 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.897 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.160 |
| High resolution limit [Å] | 2.120 | 5.750 | 2.120 |
| Rmerge | 0.087 | 0.048 | 0.523 |
| Number of reflections | 41285 | ||
| <I/σ(I)> | 11 | ||
| Completeness [%] | 99.0 | 91.8 | 99.9 |
| Redundancy | 4.5 | 4.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 277 | PEG 4000, Sodium citrate, Glycerol, pH 4.2, vapor diffusion, temperature 277K |
