4KZF
The mechanism of the amidases: The effect of the mutation E142L in the amidase from Geobacillus pallidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-28 |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 130.979, 130.979, 130.979 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.300 - 1.850 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2plq |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.941 |
| Data reduction software | d*TREK |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 92.620 |
| High resolution limit [Å] | 1.840 |
| Rmerge | 0.080 |
| Number of reflections | 33703 |
| <I/σ(I)> | 10.6 |
| Completeness [%] | 99.7 |
| Redundancy | 6.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 1.2M sodium citrate, 0.4M sodium chloride, 0.1M sodium acetate, pH 5.6, vapor diffusion, hanging drop, temperature 294K |
