4KZE
Crystal structure of an RNA aptamer in complex with Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-04-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.941, 80.104, 94.519 |
Unit cell angles | 90.00, 111.86, 90.00 |
Refinement procedure
Resolution | 45.366 - 2.404 |
R-factor | 0.1884 |
Rwork | 0.186 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | RAPD |
Phasing software | RAPD |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.370 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.066 | 0.536 |
Number of reflections | 38273 | |
<I/σ(I)> | 1.9 | |
Completeness [%] | 96.5 | 95.3 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 8% Tacsimate, 20% PEG 3,350, 0.1 M HEPES, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.2 |