4KZD
Crystal structure of an RNA aptamer in complex with fluorophore and Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-02-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.425, 79.602, 95.210 |
| Unit cell angles | 90.00, 111.65, 90.00 |
Refinement procedure
| Resolution | 46.687 - 2.186 |
| R-factor | 0.1803 |
| Rwork | 0.179 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ivk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.168 |
| Data reduction software | HKL-2000 |
| Data scaling software | RAPD |
| Phasing software | RAPD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.690 | 2.300 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.059 | 0.615 |
| Number of reflections | 52477 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 98.2 | 98.3 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% Tacsimate, 20% PEG 3,350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
