4KZ2

Crystal Structure of phi29 pRNA 3WJ Core

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98789
Spacegroup name	I 4
Unit cell lengths	125.012, 125.012, 26.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.050
R-factor	0.182
Rwork	0.197
R-free	0.19700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7 base-pair A-form helix
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	88.400
High resolution limit [Å]	3.050
Rmerge	0.088
Number of reflections	4203
<I/σ(I)>	30.3
Completeness [%]	99.7
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	295	100mM Sodium acetate, 24-26% MPD, 20mM CaCl2, 10mM MnCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K