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4KYY

Crystal Structure of RNA 17-mer UUCGGUUUUGAUCCGGA duplex

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X25
Synchrotron siteNSLS
BeamlineX25
Temperature [K]130
Detector technologyPIXEL
Collection date2013-05-03
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.1
Spacegroup nameP 31 2 1
Unit cell lengths44.010, 44.010, 91.670
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution35.193 - 2.350
R-factor0.1994
Rwork0.195
R-free0.23930
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)UUCGG RNA duplex idealized structure
RMSD bond length0.004
RMSD bond angle0.776
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHENIX
Refinement softwarePHENIX ((phenix.refine: 1.8.1_1168))
Data quality characteristics
 Overall
Low resolution limit [Å]99.000
High resolution limit [Å]2.350
Number of reflections4651
<I/σ(I)>20.81
Completeness [%]99.8
Redundancy9.1
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP72940.2M KF, 40%(w/v) hexelene glycol (MPD), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K

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