4KYY

Crystal Structure of RNA 17-mer UUCGGUUUUGAUCCGGA duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	130
Detector technology	PIXEL
Collection date	2013-05-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 31 2 1
Unit cell lengths	44.010, 44.010, 91.670
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.193 - 2.350
R-factor	0.1994
Rwork	0.195
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UUCGG RNA duplex idealized structure
RMSD bond length	0.004
RMSD bond angle	0.776
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	99.000
High resolution limit [Å]	2.350
Number of reflections	4651
<I/σ(I)>	20.81
Completeness [%]	99.8
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294	0.2M KF, 40%(w/v) hexelene glycol (MPD), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K