4KYY
Crystal Structure of RNA 17-mer UUCGGUUUUGAUCCGGA duplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 130 |
Detector technology | PIXEL |
Collection date | 2013-05-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 44.010, 44.010, 91.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.193 - 2.350 |
R-factor | 0.1994 |
Rwork | 0.195 |
R-free | 0.23930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | UUCGG RNA duplex idealized structure |
RMSD bond length | 0.004 |
RMSD bond angle | 0.776 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.350 |
Number of reflections | 4651 |
<I/σ(I)> | 20.81 |
Completeness [%] | 99.8 |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 0.2M KF, 40%(w/v) hexelene glycol (MPD), pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |